|
RBRC No. |
RBRC01723
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
CAD Knockout mouse |
|
Former Common name |
CAD KO |
|
H-2 Haplotype |
No Data |
|
Donor strain |
(129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2003. A neomycin cassette replaced exons 1-6 and part of 7 of Dffb gene. The mutant mice were backcrossed to C57BL/6. |
|
Strain description |
B6;129-Dffb<tm1Osa>. CAD, Caspase-activated DNase is an endonuclease that is activated in apoptotic cells. CAD deficient thymocytes did not undergo apoptotic DNA fragmentation, but their DNA was digested by thymic macrophages that effectively phagocytosed the
apoptotic thymocytes. This strain is useful for elucidation of the function of CAD gene. |
|
Colony maintenance |
Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Homozygous mutant mice show no gross developmental abnormalities and are fertile with normal fecundity. |
|
Animal health report |
No Data |
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Dffb |
| Symbol name |
DNA fragmentation factor, beta subunit |
| Chromosome |
4 |
| Common name |
40kDa, 5730477D02Rik, caspase-activated DNase, CPAN, DFF40, Didff |
| Symbol description |
No Data |
|
Promoter |
herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
| 2 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
4 |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
|
References |
Nat Immunol. 2003 Feb;4(2):138-44. Epub 2003 Jan 13. Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation. |
|
Research applications |
No Data |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. immunol., 4, 138-144 (2003).
RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
|
Additional information |
|
1 |
Lab HP |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Nagata, Shigekazu (Osaka University Graduate School of Medicine, Faculty Medicine)  |
|
Strain Status /
Availability
(Expected delivery) |
|
