Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 20, 2018
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC02945  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name ICR.Cg-Tg(Thy1-ECFP,-EYFP)1Hsak  
Former Common name Thy1-stop-syn::ECFP-ires-gEYFP  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Ko Kobayakawa, Reiko Hayashi and Hitoshi Sakano, School of Science The University of Tokyo in 2002. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.  
Strain description Thy1-stop-syn::CFP-ires-gapEYFP mice having a transgene of a Thy1 promoter, loxP-STOP loxP cassette, synaptotagmin-CFP and ires-gapEYFP. Tissue-specific expression of Cre recombinase has not been examined yet.  
Colony maintenance Carrier x Noncarrier [or Crossing to Crlj:CD1(ICR)]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol No Data  
Symbol name mouse gap43 palmitoylation signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol No Data  
Symbol name mouse synaptotagmin I (Syt1)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol EYFP  
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 5 Symbol His3  
Symbol name His3 (S. cerevisiae)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter mouse Thy1 promoter  
 7 Symbol ECFP  
Symbol name Enhanced Cyan Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cell Biology Research,
Fluorescent Proteins/lacZ System,
Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER (Ko KOBAYAKAWA : skobayak@mail.ecc.u-tokyo.ac.jp). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
Depositor Sakano, Hitoshi (The University of Tokyo)  Sakano, Hitoshi 
Strain Status /
Availability
(Expected delivery)

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