Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 20, 2018
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
Figure
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RBRC No. RBRC05153  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S6-Gt(ROSA)26Sor<tm1(CAG-EGFP/TeNT)Imayo>  
Former Common name R26-CAG-LF-EGFP-TeNT  
H-2 Haplotype No Data  
ES cell line TC1 [129S6/SvEvTac]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description EGFP-TeNTpest is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. EGFP-TeNTpest is expressed under CAG promoter.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol Odc1  
Symbol name mouse ornithine decarboxylase (PEST sequence) cDNA  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 5 Symbol TeNT  
Symbol name tetanus neurotoxin light chain (Clostridium tetani)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 7 Symbol poly A  
Symbol name poly A  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 8 Symbol puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The Journal of Neuroscience, 34, 5788-5799 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP  
 2 Genotyping protocol <PCR>  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
Availability
(Expected delivery)

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