Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jul 20, 2018
Health Report   PCR Protocol   Thawing Method
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RBRC No. RBRC05164  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-GCaMP3)Imayo>  
Former Common name R26-CAG-FRT-GCaMP3  
H-2 Haplotype No Data  
ES cell line TC1 [129S6/SvEvTac]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain description GCaMP3 is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, GCaMP3 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 2 Symbol GCaMP3  
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA  
Chromosome 6  
Common name No Data  
Symbol description No Data  
References   
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Rep., 9(5):1896-1907 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.  
Additional information
 1 Dr. Loren Looger, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP  
 2 Genotyping protocol <PCR>  
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University)  Imayoshi, Itaru 
Strain Status /
Availability
(Expected delivery)

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