|
RBRC No. |
RBRC00109
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
C3H/HeN-TgH(Zic1) |
|
Former Common name |
Zic1 KO (C3H background) |
|
H-2 Haplotype |
No Data |
|
ES cell line |
J1 [129/Sv](D3 subline) |
|
Donor strain |
B6;129S4-Zic1tm1Jaru |
|
Background strain |
C3H/HeN |
|
Appearance |
| 1 |
Appearance |
agouti |
| Genotype |
No Data |
|
Strain development |
Developed by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations. |
|
Strain description |
The Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic! exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.
Genotype of Affected Mutation:Zic1 null heterozygous. |
|
Colony maintenance |
Backcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs. |
|
Animal health report |
No Data |
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Zic1 |
| Symbol name |
zinc finger protein of the cerebellum 1 |
| Chromosome |
9 |
| Common name |
zinc finger protein of the cerebellum 1 |
| Symbol description |
No Data |
|
Promoter |
mouse phosphoglycerate kinase promoter (PGK promoter) |
| 2 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
9 |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
|
References |
Brain Res Mol Brain Res. 2000 May 31;78(1-2):15-25.
Dev Biol. 2002 Apr 15;244(2):329-41.
J Neurosci. 1998 Jan 1;18(1):284-93.
Mech Dev. 1999 Dec;89(1-2):141-50. A 5' segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5' segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5' segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5' segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord. |
|
Research applications |
Analysis of abnormal behavior,
Analysis of cerebellar development,
Developmental Biology Research,
It can be regarded as a model of human Joubert Syndrome. ogura et al. Behav. Genet. 31, 317-324 (2001).,
Mouse Models for Human Disease,
Neurobiology Research |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 18, 284-293 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
|
Additional information |
|
1 |
Lab HP |
|
Depositor |
Aruga, Jun (RIKEN Brain Science Institute)  |
|
Strain Status /
Availability
(Expected delivery) |
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