Strain Data Sheet
RBRC00158
Strain Information | |
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| Image | |
| BRC No. | RBRC00158 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(gpt delta)1Nmi/Rbrc |
| Former Common name | C57BL/6-TgN(gpt Delta) |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6 |
| Appearance | black [a/a] |
| Strain development | This strain was developed for efficient detection of mutations in vivo by Dr.Nohmi T. |
| Strain description | This strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli. |
| Colony maintenance | |
| References | Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728 Cancer Res., 61, 3913-3918 (2001). 11358805 Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717 Cancer Lett., 143, 241-244 (1999). 10503911 Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079 Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718 Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358 Carcinogenesis, 21, 2049-2056 (2000). 11062167 Environ. Mutagen Res., 22, 85-90 (2000). |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)17 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter gpt Deltaguanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>transgene insertion 1, Takehito Nohmi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter red/gamlambda phage red/gam17red/gam |
Ordering Information | |
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| Donor DNA | lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC |
| Research application | |
| Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
| Depositor | Takehiko Noumi (National Institute of Health Sciences) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |