Strain Information | |
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Image | |
BRC No. | RBRC00752 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Cd40<tm1Kik>/Rbrc |
Former Common name | CD40 Knockout mice, B6.129P2-CD40<tm1Kik>/Rbrc, B6.129P2-Tnfrsf5<tm1Kik>/Rbrc |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hitoshi Kikutani, Institute for Molecular and Cellular Biology, Osaka University in 1994. |
Strain description | CD40 knockout mice. Exon 3 of the CD40 was replaced with a neo cassette. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation. Homozygous mutant mice for CD40 targeted mutation exhibit impaired immunoglobulin class switching, and the germinal center formation was defective. Homozygous mice are viable and fertile. |
Colony maintenance | |
References | Immunity, 1, 167-178 (1994). 7534202 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)2neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Tnfrsf5 genomic DNA |
Research application | Immunology and Inflammation Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 1, 167-178 (1994). |
Depositor | Hitoshi Kikutani (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |