Strain Data Sheet

RBRC00760

Strain Information

Image
BRC No.RBRC00760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Gjc1<tm1Kni>/KniRbrc
Former Common nameB6;129S-Gja7<tm1Kni>/KniRbrc, B6;129S-Gjc1<tm1Kni>/KniRbrc, Cx45 Knockout mice
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDevelopment, 127, 3501-3512 (2000). 10903175

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gjc1gap junction membrane channel protein alpha 711Gjc1<tm1Kni>targeted mutation 1, Kiyomasa Nishiiconnexin45(Cx45) ; Cnx45, connexin 45, Cx45, Gja-7, Gja7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Ordering Information

Donor DNAmouse connexin45(Cx45) genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), mouse phosphoglycerate kinase promoter (PGK promoter), Phage P1 loxP, E. coli neo, E. coli lacZ
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 127, 3501-3512 (2000).
DepositorKiyomasa Nishii (National Defense Medical College)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data