Strain Data Sheet
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Data update: Oct 24, 2017
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RBRC No. RBRC00806  
Type TransgeneCartagena  
Species Mus musculus  
Strain name GFP-LC3#53  
Former Common name No Data  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
Appearance
 1 Appearance black  
Genotype No Data  
Strain development Developed by Noboru Mizushima, National Institute for Basic Biology. The transgene was injected into the pronuclei of C57BL/6 x (C57BL/6 x DBA/2)F1 fertilized eggs. The mice were backcrossed to C57BL/6J.  
Strain description These transgenic mice express a fusion cDNA encoding enhanced GFP jointed at its C-terminus to rat microtubule-associated protein 1 light chain 3 beta under the control of the CAG promoter. The occurrence of autophagy in mouse tissues can be monitored by the GFP.  
Colony maintenance Carrier x Carrier, Noncarrier [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Map1lc3b  
Symbol name microtubule-associated protein 1 light (rat)  
Chromosome UN  
Common name microtubule associating protein (MAP)1 light chain 3  
Symbol description LC3 is the first mammalian protein identified to associate with autophagosomes.
It is a mammalian homologue of yeast Aut7/Apg8, and is required for autophagosome formation.  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Mol Biol Cell. 2004 Mar;15(3):1101-11. Epub 2003 Dec 29.
Nature. 2004 Dec 23;432(7020):1032-6. Epub 2004 Nov 3.  














Research applications Cell Biology Research,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Biol. Cell 15, 1101-1111 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP  
 2 Mouse of the Month Mar 2005
Mouse of the Month Mar 2012
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Mizushima, Noboru (The University of Tokyo, Graduate School of Medicine)  Mizushima, Noboru 
Strain Status /
Availability
(Expected delivery)

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