Strain Information | |
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Image | |
BRC No. | RBRC00808 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Emx1<tm1(cre)Ito>/ItoRbrc |
Former Common name | Emx1-Cre |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | black (a/a B/B C/C) |
Strain development | Developed by Takuji Iwasato and Shigeyoshi Itohara, RIKEN Brain Science Institute. E14 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6J. |
Strain description | Emx1-cre knock-in mice. neo type (inserted a cre recombinase gene and a frt flanked PGK-neo cassette into Emx1 gene)(RBRC00808), delta neo type (removed the pgk-neo cassette by FLP mediated recombination)(RBRC01345). The expression of cre recombinase was observed in dorsal telencephalon, neocortex, hippocampus, and olfactory bulb. In the delta neo type (RBRC01345), no ectopic cre expression was detected. Homozygous mice are viable and fertile. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Nature, 406, 726-731 (2000). 10963597 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Emx1empty spiracles homolog 1 (Drosophila)6Emx1<tm1(cre)Ito>targeted mutation 1 (cre), Shigeyoshi Itohara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase6cremouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)6nls Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, SV40 poly A, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Emx1 (Drosophila homeobox gene empty spiracles (Ems) mouse homolog) genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 406, 726-731 (2000). |
Depositor | Shigeyoshi Itohara (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month June 2006 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Imayoshi I, Shimogori T, Ohtsuka T, Kageyama R. Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline. Development 135(15) 2531-41(2008) 18579678 Kita A, Imayoshi I, Hojo M, Kitagawa M, Kokubu H, Ohsawa R, Ohtsuka T, Kageyama R, Hashimoto N. Hes1 and Hes5 control the progenitor pool, intermediate lobe specification, and posterior lobe formation in the pituitary development. Mol Endocrinol 21(6) 1458-66(2007) 17426285 Kinameri E, Inoue T, Aruga J, Imayoshi I, Kageyama R, Shimogori T, Moore AW. Prdm proto-oncogene transcription factor family expression and interaction with the Notch-Hes pathway in mouse neurogenesis. PLoS One 3(12) e3859(2008) 19050759 |