Strain Data Sheet

RBRC00866

Strain Information

Image
BRC No.RBRC00866
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-NR4A3)N2/Rbrc
Former Common nameC57BL/6-TgN(act-Nor-1)N2
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Genox Research, Inc. in 2002. The transgene was injected into the pronucleous of C57BL/6N fertilized eggs.
Strain descriptionThese transgenic mice express human NOR-1 for neuron derived orphan receptor, (GenBank accesion No. D78579) under the control of the CAG promoter. Expression level of the transgene in brain is high in line N1 (RBRC00865), subsequently in line N3 (RBRC00867), less in line N2 (RBRC00866). Expression of the transgene in spleen was also observed in line N1.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
NR4A3neuron derived orphan receptor (human)UNNR4A3neuron derived orphan receptor, human Nor-1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attB1attachment siteUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attB2attachment siteUN

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Human NOR-1 cDNA for neuron derived orphan receptor, attB1, attB2
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorRyoichi Hashida (Eisai Co., Ltd.)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data