Strain Data Sheet

RBRC00951

Strain Information

Image
BRC No.RBRC00951
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Psen1<tm1Shs>/Shs
Former Common namePsen1 null
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Takuji Sirasawa, Tokyo Metropolitan Institute of Gerontology at 2002. A tk-neo cassette from pMC1-neo was inserted into the exon 9 of the Psen1 gene.
Strain descriptionB6.Cg-Psen1<tm1Shs>. Presenilin 1 has been found as a major causal gene for early-onset familial Alzheimer's disease (FAD), involving in the control of the cell cycle in neuronal precursor cells during development. Homozygous mutant embryos exhibit small body size and intracranial hemorrhages at late gestation.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
ReferencesJ. Neurosci. Res., 70(3):501-13 (2002). 12391611
Development, 128, 1391-1402 (2001). 11262239

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Psen1presenilin 112Psen1<tm1Shs>targeted mutation 1, Takuji ShirasawaAd3h, presenilin-1, PS-1, PS1, S182

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse presenilin 1 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Koizumi et al, Development, 128, 1391-1402 (2001). The users must collaborate with the supplier for two years from the date of supply.
The users must discuss with the supplier when the users propose patents or present findings based on the results of investigation using the resource.
DepositorTakuji Shirasawa (Tokyo Metropolitan Institute of Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese), Lab HP (English)
Genotyping protocol -PCR-

BRC mice in Publications

No Data