Strain Information | |
---|---|
Image | |
BRC No. | RBRC00961 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129P2-Gal3st1<tm1Kho>/KhoRbrc |
Former Common name | C57BL/6N x CST KO-1(hetero), TgH(CSTneo) 01 |
H-2 Haplotype | |
ES Cell line | E14.1 [129P2/OlaHsd] |
Background strain | C57BL/6NJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Koichi Honke, Osaka University Medical School in 1999. A targeting vector including pgk-neo was transfected into E14.1 ES cells to disrupt exons 2 and 3 of Cst gene. The Cst knockout mice were backcrossed to C57BL/6N for 6 times. Generation: N5+N1F3 |
Strain description | B6;129P2-Gal3st1<tm1Kho>. Cerebroside sulfotransferase (Cst) gene is located in the Golgi membrane that is responsible for the biosynthesis of two sulfated glycolipids (GalCer and GalEAG) in the mammals. Cst null mice lack sulfatide in myelin and sulfated seminolipid in sperm. Homozygous mutant males are sterile. This strain is useful for elucidation of the role of sulfoglycolipids in myelin function and spermatogenesis. |
Colony maintenance | |
References | J. Biol. Chem., 279, 2085-2090 (2004). 14583626 Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). 11917099 Glia, 45, 269-277 (2004). 14730700 J. Neurosci., 22, 6507-6514 (2002). 12151530 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gal3st1galactosylceramide sulfotransferase11Gal3st1<tm1Kho>targeted mutation 1, Koichi Honke3'-phosphoadenylylsulfate-galactosylceramide 3'-sulfotransferase, CST, galactosylceramide sulfotransferase, GalCer sulfotransferase Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
---|---|
Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Gal3st1 genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). |
Depositor | Koichi Honke (Kochi University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
Zhang Y, Hayashi Y, Cheng X, Watanabe T, Wang X, Taniguchi N, Honke K. Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis. Glycobiology 15(6) 649-54(2005) 15659616 |