Strain Data Sheet
RBRC00980
Strain Information | |
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| Image | |
| BRC No. | RBRC00980 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6C3F1-Tg(CAG-EGFP)CX-FM038Osb |
| Former Common name | 152A; B6C3F1B6C3F1-TgN(act-EGFP)OsbCX-FM038 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | Slc:B6C3F1 |
| Appearance | black or agouti [a/a B/B C/C or A/? B/B C/C] |
| Strain development | Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs. |
| Strain description | The CAG-EGFP transgene is located on X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies. |
| Colony maintenance | Sibling Mating |
| References | Curr. Biol., 16(2):166-172 (2006). 16431368 FEBS Lett., 407, 313-319 (1997). 9175875 Genomics, 80, 564-574 (2002). 12504848 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPGreen Fluorescent Protein (Aequorea victoria)XEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genomics, 80, 564-574 (2002). Curr. Biol., 16(2):166-172 (2006).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masaru Okabe (Osaka University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M. The X-linked imprinted gene family Fthl17 shows predominantly female expression following the two-cell stage in mouse embryos. Nucleic Acids Res 38(11) 3672-81(2010) 20185572 Watanabe-Takano H, Takano K, Sakamoto A, Matsumoto K, Tokuhisa T, Endo T, Hatano M. DA-Raf-dependent inhibition of the Ras-ERK signaling pathway in type 2 alveolar epithelial cells controls alveolar formation. Proc Natl Acad Sci U S A 111(22) E2291-300(2014) 24843139 |