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RBRC No. |
RBRC01099
|
|
Type |
Transgene |
 |
|
Species |
Mus musculus |
|
Strain name |
ERAI mice |
|
Former Common name |
ER stress-activated indicator (ERAI) |
|
H-2 Haplotype |
No Data |
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Background strain |
C57BL/6 |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Takao Iwawaki and Masayuki Miura, RIKEN Brain Science Institute. The 3.6-kb fragment of pCAX-F-XBP1deltaDBD-venus was injected into the pronuclei of C57BL/6 fertilized eggs. Transgenic mice were backcrossed to C57BL/6JJcl. |
|
Strain description |
ERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo. |
|
Colony maintenance |
Carrier x Noncarrier [C57BL/6JJcl]. Tg mice are viable and fertile. |
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Animal health report |
|
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Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
XBP1 |
| Symbol name |
X-box binding protein 1 (human) |
| Chromosome |
UN |
| Common name |
D11Ertd39e, TREB-5, TREB5, TREB5, XBP-1 |
| Symbol description |
No Data |
| 2 |
Symbol |
Venus |
| Symbol name |
Green Fluorescent Protein, F46L |
| Chromosome |
UN |
| Common name |
Venus |
| Symbol description |
No Data |
|
Promoter |
CMV-IE enhancer, chicken beta-actin promoter |
| 3 |
Symbol |
FLAG |
| Symbol name |
FLAG tag (synthetic) |
| Chromosome |
UN |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
Nat Med. 2004 Jan;10(1):98-102. Epub 2003 Dec 14. Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1.
Airway epithelial inflammation-induced endoplasmic reticulum Ca2+ store expansion is mediated by X-box binding protein-1. |
|
Research applications |
Cell Biology Research,
Fluorescent Proteins/lacZ System |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Med., 10, 98-102 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
During 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR, and to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA. If there is intent to use the material for profit purpose, the recipient shall first discuss its intentions with RIKEN. The recipient shall agree to acknowledge RIKEN in all publications with regard to the material. |
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Additional information |
|
1 |
Lab HP (Japanese) |
|
2 |
Mouse of the Month Mar 2007
Genetic Background |
|
3 |
Genotyping protocol <PCR> |
|
Depositor |
Miura, Masayuki (The University of Tokyo)  |
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Strain Status /
Availability
(Expected delivery) |
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