Strain Data Sheet
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Data update: Feb 21, 2014
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RBRC No. RBRC01122  
Type Transgene CongenicCartagena   wks
Species Mus musculus  
Strain name hRN 8-12  
Former Common name No Data  
H-2 Haplotype No Data  
Background strain C57BL/6J  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Akiyoshi Fukamizu, University of Tsukuba in 1991. C57BL/6 background.  
Strain description Human renin gene transgenic mice with integration of 10 copies of a 15 kbp human DNA fragment containing the renin gene (hRN 8-12 mice). The renin-angiotensin system (RAS) is one of the determinant factors in regulating blood pressure and sodium absorption. RAS has been shown to accelerate various diseases such as renal failure, atherosclerosis, cardiac hypertrophy, and pulmonary hypertension. The reaction between renin, produced in the kidney, and its substrate angiotensinogen, produced in the liver, is the initial and rate-limiting step in the enzymatic cascade that generates angiotensin I. Angiotensin-converting enzyme in the lung converts angiotensin I to the potent vasoconstrictor angiotensin II (Ang II). The depositor reported that overactivation of the RAS induces hypertension in the F1 generation obtained from breeding between a female carrying the human renin gene (hRN+/+) and a male possessing the human angiotensinogen gene (hAG+/+). Moreover, female hAG+/+ mice when bred with male hRN+/+ mice are hypertensive specifically in late pregnancy, suggesting possible implications for preeclampsia or pregnancy-induced hypertension, a life-threatening disorder for human mothers and fetuses. hRN+/+ and hANG+/+ mice provide the opportunity to understand the role of the RAS in hypertension and other common diseases. hRN8-12 mice (RBRC01122), hAG 2-5 mice (RBRC01123), hRN8-12 x hAG2-5)F1 mice (RBRC02432).  
Colony maintenance Carrier x Carrier (Homozygote x Homozygote) Homozygous mice are viable and fertile.  
Health Certificate
Gene Details
Promoter No Data  
 1 Symbol REN1  
Symbol name renin 1 structural (human)  
Chromosome UN  
Common name Ren, Ren-1, Ren-A, Ren1d, Rn-1, Rnr  
Symbol description No Data  
References Biochem Biophys Res Commun. 1989 Dec 15;165(2):826-32.  
Tissue-specific expression of the human renin gene in transgenic mice.
Research applications Cell Biology Research,
Hematological Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochemical and Biophysical Research Communications, 165, 826-832 (1989).
Within a period of 2 years after deposition of the material, user must contact bailor and notify him a content of the research. If the bailor judges that the research should be a collaborative work, then user must include bailor and/or developer's names as co-authors.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month May 2012
Systolic blood pressure
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Fukamizu, Akiyoshi (University of Tsukuba)  Fukamizu, Akiyoshi 
Strain Status /
Availability
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