Strain Information | |
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Image | |
BRC No. | RBRC01145 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | ICR.Cg-Mesp1<tm2(cre)Ysa>/YsaRbrc |
Former Common name | Mesp1-cre |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | ICR MCH |
Appearance | Agouti or black [A/? or a/a B/B Mesp1-Cre c/+ C] albino [A/? or a/a B/B + c/+ C] |
Strain development | Developed by Yumiko Sga, National Institute of Health Sciences in 1998. A Cre gene and pgk-neo cassette were ligated in frame at a NcoI site located at the translational initiation site of Mesp1 gene. ICR mixed background. |
Strain description | Mesp1-Cre knock-in mice, in which Cre recombinase is expressed under the control of the endogenous promoter-enhancer. By crossing with a mouse strain with a reporter gene like a GFP and LacZ which is interrupted by a floxed CAT gene, it is possible to detect Mesp1-expressing cells. Mesp1 is a transcriptional factor temporarily expressing in endothelium of the blood vessel in a part of lateral plate mesoderm and heart cells, and is a determinant that is important for the formation of heart. This strain is useful for understanding of the mechanisms underlying cardiovascular development. Homozygous mutant mice are embryonic lethal. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]. Homozygous mutant mice are embryonic lethal. |
References | Development, 126, 3437-3447 (1999). 10393122 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase7creMesp1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Mesp1 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 126, 3437-3447 (1999). |
Depositor | Yumiko Saga (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2006 Genetic Background |
BRC mice in Publications |
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Funato N, Heliövaara A, Boeckx C. A regulatory variant impacting TBX1 expression contributes to basicranial morphology in Homo sapiens. Am J Hum Genet (2024) 38608674 MacDonald ST, Bamforth SD, Chen CM, Farthing CR, Franklyn A, Broadbent C, Schneider JE, Saga Y, Lewandoski M, Bhattacharya S. Epiblastic Cited2 deficiency results in cardiac phenotypic heterogeneity and provides a mechanism for haploinsufficiency. Cardiovasc Res 79(3) 448-57(2008) 18440989 Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell 3(1) 55-68(2008) 18593559 Thompson H, Ohazama A, Sharpe PT, Tucker AS. The origin of the stapes and relationship to the otic capsule and oval window. Dev Dyn 241(9) 1396-404(2012) 22778034 Rothova M, Thompson H, Lickert H, Tucker AS. Lineage tracing of the endoderm during oral development. Dev Dyn 241(7) 1183-91(2012) 22581563 Funato N, Nakamura M, Richardson JA, Srivastava D, Yanagisawa H. Loss of Tbx1 induces bone phenotypes similar to cleidocranial dysplasia. Hum Mol Genet 24(2) 424-35(2015) 25209980 Randall V, McCue K, Roberts C, Kyriakopoulou V, Beddow S, Barrett AN, Vitelli F, Prescott K, Shaw-Smith C, Devriendt K, Bosman E, Steffes G, Steel KP, Simrick S, Basson MA, Illingworth E, Scambler PJ. Great vessel development requires biallelic expression of Chd7 and Tbx1 in pharyngeal ectoderm in mice. J Clin Invest 119(11) 3301-10(2009) 19855134 Funato N, Srivastava D, Shibata S, Yanagisawa H. TBX1 Regulates Chondrocyte Maturation in the Spheno-occipital Synchondrosis. J Dent Res 99(10) 1182-1191(2020) 32442036 Domínguez-Frutos E, Vendrell V, Alvarez Y, Zelarayan LC, López-Hernández I, Ros M, Schimmang T. Tissue-specific requirements for FGF8 during early inner ear development. Mech Dev 126(10) 873-81(2009) 19619645 |