Strain Information | |
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Image | |
BRC No. | RBRC01298 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(CAT-Rho-K DN)5-18Koba/KobaRbrc |
Former Common name | CAT-Rho-K DN/5-18 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6J |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. |
Strain description | These transgenic mice express a dominant-negative form for RhoA for Rho-kinase (Rho-K DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Copy number: line 3-1 (RBRC01294)_10 copies, line 5-18 (RBRC01298)_1 copy. |
Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] Tg mice are viable and fertile. |
References | J. Neurosci., 24, 3480-3488 (2004). 15071095 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Rock2Rho-associated coiled-coil containing protein kinase 2 (bovine)UNRock2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter catE. coli chloramphenicol acetyltransferase (CAT)UNCMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP |
Ordering Information | |
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Donor DNA | CMV promoter, rabbit beta-globin intron, E. coli chloramphenicol acetyltransferase (CAT) gene, bovine Rho-K DN cDNA, loxP, rabbit beta-globin poly A, SV40 early gene poly A |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neursci., 24, 3480-3488 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Kazuto Kobayashi (Fukushima Medical University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Jun 2005 |
BRC mice in Publications |
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