Strain Data Sheet
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Data update: Nov 22, 2017
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RBRC No. RBRC01390  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129P2-Nfe2l2<tm1Mym>/MymRbrc  
Former Common name Nrf2 knockout mouse/C57BL6J  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Donor strain 129P2/OlaHsd via E14 ES cell line  
Background strain C57BL/6  
Appearance
 1 Appearance Black  
Genotype a/a B/B C/C  
Strain development Developed by Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 1996. The targeting vector containing lacZ-neo cassette was transferred into E14 ES cells to replace the 1.2 kb segment containing the rest of the exon 5 coding sequence of the Nrf2 gene.  
Strain description Nrf2 (Nfe2l2) Knockout mice. This strain is a useful model for the in vivo analysis of chemical carcinogenesis and resistance to anti-cancer drugs. A phenolic antioxidant significantly induced the expression of phase II enzymes such as glutathione S-transferase and NAD(P)H: quinone oxidoreductase in normal mice. However, in the homozygous Nrf2 deficient mice the induction of the phase II enzymes by a phenolic antioxidant was largely eliminated in the liver and intestine. Since Nrf2 is constantly degraded by proteasome under normal condition, it is hardly to detect Nrf2 protein in the absence of stimuli. Electrophilic chemicals, such as DEM (diethyl maleate) and tBHQ (tert-buthyl hydroquinone), are generally used to stabilize Nrf2. Or proteasomal inhibitors, such as MG132, are also effective increasing the protein amount of Nrf2. C57BL/6 background (RBRC01390), BALB/c background (RBRC02190), A/J background (RBRC02414), ICR mixed background (RBRC00984). Homozygous mutant mice are viable and fertile, but show lower reproductive performance.  
Colony maintenance Homozygote x Heterozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Nfe2l2  
Symbol name nuclear factor, erythroid derived 2, like 2  
Chromosome 2  
Common name Nrf2  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 2  
Common name No Data  
Symbol description E. coli beta galactosidase  
 3 Symbol nls  
Symbol name SV40 large T antigen nuclear localization signal (NLS)  
Chromosome 2  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 2  
Common name neo; neomycin;  
Symbol description No Data  
References Biochem Biophys Res Commun. 1997 Jul 18;236(2):313-22.  
Induction of sulfiredoxin via an Nrf2-dependent pathway and hyperoxidation of peroxiredoxin III in the lungs of mice exposed to hyperoxia.
Induction of sulfiredoxin via an Nrf2-dependent pathway and hyperoxidation of peroxiredoxin III in the lungs of mice exposed to hyperoxia.
Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 236, 313-322 (1997).
The RECIPIENT agrees that it will acknowledge the DEPOSITOR as academically and scientifically appropriate in its articles when the Recipient publishes its data using these mice. Recipient should contact the DEPOSITOR in the case of application for any patents with the results from these mice.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Oct 2005
Mouse of the Month Apr 2012
Genetic Background
Breeding characters  
 3 Genotyping protocol <PCR>  
Depositor Yamamoto, Masayuki (University of tsukuba)  Yamamoto, Masayuki 
Strain Status /
Availability
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