Strain Information | |
---|---|
Image | |
BRC No. | RBRC01400 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6D2-Tg(CAG-Ptafr)1Tksh/TkshRbrc |
Former Common name | PAFR-Tg, Platelet-activating factor (PAF) receptor transgenic mouse |
H-2 Haplotype | |
ES Cell line | |
Background strain | Crlj:B6D2F1 |
Appearance | |
Strain development | Developed by Dr. Satoshi Ishii at the University of Tokyo in 1997. A transgenic construct containing a guinea-pig PAFR gene under the control of the a chiken beta actin promotor and the CMV immediate early enhancer, was introduced into the pronuclei of fertilized eggs of B6D2F2. The transgenic mice were mated with B6D2F1. |
Strain description | Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. This transgenic mice showed a bronchial hyperreactivity, increased endotoxin lethality and melanocytic tumorigenesis. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo. |
Colony maintenance | Cross to B6D2F1 (Hemizygote x Crj:B6D2F1) |
References | EMBO J., 16, 133-142 (1997). 9009274 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ptafrplatelet-activating factor receptor (guinea-pig)UNPtafrPAF receptor, PAFRCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
---|---|
Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), guinea-pig Platelet-activating factor (PAF) receptor cDNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J., 16, 133-142 (1997). |
Depositor | Takao Shimizu (The University of Tokyo) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |