Strain Information | |
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Image | |
BRC No. | RBRC01464 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(Cg)-Cacna1g<tm1.1Ksak>/KsakRbrc |
Former Common name | Ca1G(flox), C57BL/6-Cacna1g<flox/+> |
H-2 Haplotype | |
ES Cell line | Bruce (gifted by Dr. Colin L. Stewart) |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Kenji Sakimura, Brain Research Institute, Nigata University in 2002. The targeted allele of this strain was flanked exon 5 with loxP sites and a FRT flanked Neo resistant gene driven by the PGK promotor. The FRT-flanked neo was excised by crossing with mice expressing Flp. |
Strain description | Cacna1g gene is a member of the low-voltage-activated calcium channel that plays a critcal role in calcium ion transport in nerve, muscle and endocrine cells. The targeted allele of this strain was flanked exon 5 with loxP sites. The FRT-flanked neo was excised by crossing with mice expressing Flp. Conditional Cacna1g deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | |
References | Biochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cacna1gcalcium channel, voltage-dependent, T type, alpha 1G subunit11Cacna1g<tm1.1Ksak>targeted mutation 1.1, Kenji Skimura[a]1G, a1G, alpha-1G, Cav3.1d, mKIAA1123 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site11Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, S. cerevisiae 2μ plasmid FRT (flipase recombination target) site, mouse Cacna1g genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | ・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. ・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. ・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Kenji Sakimura (Niigata University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |