Strain Data Sheet

RBRC01464

Strain Information

Image
BRC No.RBRC01464
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Cacna1g<tm1.1Ksak>/KsakRbrc
Former Common nameCa1G(flox), C57BL/6-Cacna1g<flox/+>
H-2 Haplotype
ES Cell lineBruce (gifted by Dr. Colin L. Stewart)
Background strain
Appearance
Strain developmentDeveloped by Dr. Kenji Sakimura, Brain Research Institute, Nigata University in 2002. The targeted allele of this strain was flanked exon 5 with loxP sites and a FRT flanked Neo resistant gene driven by the PGK promotor. The FRT-flanked neo was excised by crossing with mice expressing Flp.
Strain descriptionCacna1g gene is a member of the low-voltage-activated calcium channel that plays a critcal role in calcium ion transport in nerve, muscle and endocrine cells. The targeted allele of this strain was flanked exon 5 with loxP sites. The FRT-flanked neo was excised by crossing with mice expressing Flp. Conditional Cacna1g deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 293, 953-957 (2002). 12051751

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cacna1gcalcium channel, voltage-dependent, T type, alpha 1G subunit11Cacna1g<tm1.1Ksak>targeted mutation 1.1, Kenji Skimura[a]1G, a1G, alpha-1G, Cav3.1d, mKIAA1123

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Ordering Information

Donor DNAPhage P1 loxP sites, S. cerevisiae 2μ plasmid FRT (flipase recombination target) site, mouse Cacna1g genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and Conditions・The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
・The DEPOSITOR should be a co-author in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC.
・Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data