|
RBRC No. |
RBRC01492
|
|
Type |
Transgene |
 |
|
Species |
Mus musculus |
|
Strain name |
DBH-Cre/9-9 |
|
Former Common name |
No Data |
|
H-2 Haplotype |
No Data |
|
Background strain |
C57BL/6J |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Dr. Kazuto Kobayashi at Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized egges. The mice were backcrossed to C57BL/6J. |
|
Strain description |
These transgenic mice express Cre recombinase gene under the control of the human dopamin beta hydroxylase (DBH) gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in typical noradrenergic and adrenergic cell groups as well as in transient catecholaminergic cell types. |
|
Colony maintenance |
Backccross to C57BL/6 (Hemizygote x C57BL/6J)
The reproductive performance is good. |
|
Animal health report |
|
|
Gene Details |
|
Promoter |
human dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A |
| 1 |
Symbol |
cre |
| Symbol name |
Phage P1 Cre recombinase |
| Chromosome |
UN |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
J Neurosci Res. 2004 Oct 1;78(1):7-15. Fate of transient catecholaminergic cell types revealed by site-specific recombination in transgenic mice. |
|
Research applications |
Cre/loxP system |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci. Res., 78, 7-15 (2004).
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. |
|
Additional information |
|
1 |
Lab HP (Japanese) |
|
2 |
Mouse of the Month June 2006 |
|
3 |
Genotyping protocol <PCR> |
|
Depositor |
Kobayashi, Kazuto (Fukushima Medical University)  |
|
Strain Status /
Availability
(Expected delivery) |
|
