|
RBRC No. |
RBRC01516
|
|
Type |
Transgene |
 |
|
Species |
Mus musculus |
|
Strain name |
VAChT-Cre.Slow |
|
Former Common name |
No Data |
|
H-2 Haplotype |
No Data |
|
Background strain |
C57BL/6 |
|
Appearance |
| 1 |
Appearance |
black |
| Genotype |
a/a B/B C/C |
|
Strain development |
Developed by Dr. Hidemi Misawa at Tokyo Metropolitan Institute for Neuroscience in 2003. The VaChT-Cre transgene was injected into the pronuclei of fertilized oocytes of C57BL/6 mice. |
|
Strain description |
The Cre-recombinase activity was detected specifically in a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus, but absent in visceromotor and other central and peripheral cholinergic neurons. The VaChT-Cre mice promote cre-mediated recombination with late onset. |
|
Colony maintenance |
backcross to C57BL/6 (Hemizygote x C57BL/6NJcl) |
|
Animal health report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
GFP |
| Symbol name |
Green Fluorescent Protein (Jellyfish) |
| Chromosome |
UN |
| Common name |
EGFP; Egfp, enhanced green fluorescent protein; |
| Symbol description |
No Data |
| 2 |
Symbol |
IRES |
| Symbol name |
internal ribosomal entry site (EMCV) |
| Chromosome |
UN |
| Common name |
internal ribosomal entry site from EMCV |
| Symbol description |
No Data |
|
Promoter |
human vesicular acetylcholine transporter |
| 3 |
Symbol |
cre |
| Symbol name |
Phage P1 Cre recombinase |
| Chromosome |
UN |
| Common name |
No Data |
| Symbol description |
No Data |
|
References |
Genesis 2003 Sep;37(1):44-50. VAChT-Cre.Fast and VAChT-Cre.Slow: postnatal expression of Cre recombinase in somatomotor neurons with different onset. |
|
Research applications |
Cre/loxP system,
Fluorescent Proteins/lacZ System |
|
Specific Term and Conditions |
The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
A prior agreement for collaboration with the depositor should be required. |
|
Additional information |
|
1 |
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B. |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Misawa, Hidemi (Keio University Faculty of Pharmacy)  |
|
Strain Status /
Availability
(Expected delivery) |
|
