Strain Information | |
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Image | |
BRC No. | RBRC01556 |
Type | Gene Trap |
Species | Mus musculus |
Strain name | B6.Cg-Nell2<Tn(sb-lacZ,GFP)OR238Jtak>/JtakRbrc |
Former Common name | Gene trap of Nell2 (nel-like 2 homolog), OR238 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | |
Strain description | |
Colony maintenance | |
References | Nat. Methods, 2, 763-769 (2005). 16179923 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BCL2Splice acceptor (human bcl-2)15BCL2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)15CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (human)15GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA15HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)15internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SBSleeping Beauty transposon15SB Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorSplice donor (mouse hprt)15Splice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15neo; neomycin; |
Ordering Information | |
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Donor DNA | Salmon Sleeping Beauty transposon*, Salmon Sleeping Beauty transposase*, Human bcl-2 splice acceptor, Encephalomyocarditis virus (EMCV)internal ribosomal entry site (ires), E. coli LacZ gene, Rabbit beta-globin polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP gene, Mouse hprt splice donor, mouse Pgk promoter*, Mouse synaptonemal protein 1 promoter*, Human growth hormone polyadenylation signal*, pBluescript*[E. coli neomycin resistance gene was not detected by PCR. Detection of introduced genes (asterisk) were not tested.] |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Junji Takeda (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |