Strain Data Sheet

RBRC01569

Strain Information

Image
BRC No.RBRC01569
TypeGene TrapCartagena
SpeciesMus musculus
Strain nameB6.Cg-Zfp326<Tn(sb-lacZ,GFP)PV484Jtak>/JtakRbrc
Former Common namePV484
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesMol. Cell. Biol., 23, 9189-9207 (2003). 14645530
Nat. Methods, 2, 763-769 (2005). 16179923

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zfp326zinc finger protein 3265Zfp326<Tn(sb-lacZ,GFP)PV484Jtak>transposon insertion PV484, Junji Takeda5730470H14Rik, ZAN75

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)5CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)5internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor5SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorSplice donor5Splice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ApolyA5poly A

Ordering Information

Donor DNApTrans-SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), lacZ-CAG-GFP-SD: EGFP
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJunji Takeda (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Takeda Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data