Strain Information | |
---|---|
Image | |
BRC No. | RBRC01650 |
Type | Transgene |
Species | Mus musculus |
Strain name | IR2 |
Former Common name | SB transposon IR2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Junji Takeda, Graduate School of Medicine, Osaka University in 2004. |
Strain description | This transgenic transposon mouse strain has a Sleeping Beauty (SB) transposon vector acting as a mutagen. The double transgenic mice containing SB transposon vector and SB transposase, generated by crossing with this Tg mice and the Tg mice having SB transposase, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse. |
Colony maintenance | |
References | Nat. Methods, 2, 763-769 (2005). 16179923 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptorUNSA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorSplice donorUNSplice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)UNneo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls |
Ordering Information | |
---|---|
Donor DNA | pTrans-SA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), lacZ-CAG-GFP-SD: NEO, EGFP, mouse phosphoglycerate kinase promoter (PGK promoter) |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Junji Takeda (Osaka University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |