Strain Information | |
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Image | |
BRC No. | RBRC01653 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(CAG-sb10)1Jtak/JtakRbrc |
Former Common name | CAG-SB10, B6.Cg-Tg(ACTB-sb10)1Jtak |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | black |
Strain development | Developed by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2001. The CX-SB plasmid was injected into the pronuclei of B6C3F2 fertilized egges. C57BL/6, C3H and ICR mixed background. |
Strain description | This transgenic mice express Sleeping Beauty (SB) transposase under control of CAG promotor. The double transgenic mice containing SB transposase and SB transposon vector, generated by crossing with this Tg mice and the Tg mice having SB transposon vector, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse. |
Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6J) |
References | Nat. Methods, 2, 763-769 (2005). 16179923 Proc. Natl. Acad. Sci. USA, 98, 9191-9196 (2001). 11481482 Mol. Cell. Biol., 23, 9189-9207 (2003). 14645530 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SBSleeping Beauty transposaseUNSBCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), salmonid Sleeping Beauty Transposase |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Methods., 2, 763-769 (2005).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
Depositor | Junji Takeda (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |