Strain Information | |
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Image | |
BRC No. | RBRC01689 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Pcdhg<tm(EN130)>/TyagRbrc |
Former Common name | EN130, Pcdhg EN130 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Takeshi Yagi, National Institute for Physiological Sciences/Graduate School of Frontier Biosciences, Osaka University in 2001. Delta neo type (RBRC03152) was generated by crossing with Sycp-cre mice. |
Strain description | Pcdhg gene knockout mice. A polyA of Pcdhg common region was replaced with tau-lacZ gene. lacZ-neo cassette type (RBRC01689), delta neo cassette type (RBRC03152). Homozygous mice show no obvious abnormality. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] |
References | J Biol Chem. , 286, 31885-31895 (2011). 21771796 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)18internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pcdhgprotocadherin gamma cluster18Pcdhg Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)18 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)18neo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter taubovine tau protein cDNA18tau |
Ordering Information | |
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Donor DNA | mouse protocadherin gamma cDNA, P1 phage loxP, E. coli neo, E. coli lacZ, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takeshi Yagi (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |