Strain Information | |
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Image | |
BRC No. | RBRC01721 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(Emu-BCL2L1)87Nnz/Rbrc |
Former Common name | Bcl-xL Tg |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrlCrlj |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges. |
Strain description | C57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro. |
Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6NCrj). |
References | J. Exp. Med., 183, 381-391 (1996). 8627151 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter BCL2L1Bcl2-like 1UNBCL2L1bcl-x, Bcl-XL, Bcl-Xs, BclXmouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ASV40 polyA signal sequenceUNpoly A |
Ordering Information | |
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Donor DNA | mouse IgH enhancer, human Bcl-xL cDNA, SV40 early promoter and poly A (SV40-EH cassette) |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 183, 381-391 (1991).Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens). |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |