Strain Data Sheet
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Data update: Oct 24, 2017
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RBRC No. RBRC01834  
Type TransgeneCartagena  
Species Mus musculus  
Strain name C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc  
Former Common name B6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc  
H-2 Haplotype No Data  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Deposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.  
Strain description This transgenic mouse of C57BL/6 strain expresses the FLPe recombinase under the control of the CAG promoter. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37 degrees C. The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and phi C31. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.  
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 3 Symbol FLPe  
Symbol name yeast flippase recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Exp Anim. 2006 Apr;55(2):137-41.  
High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Research applications FLP/frt system,
General Purpose,
Genetics Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Experimental Animals, 55, 137-141 (2006).  
Additional information
 1 Lab HP  
 2 Mouse of the Month Feb 2007
Genetic Background  
 3 Genotyping protocol <PCR>  
Depositor Itohara, ShigeYoshi (RIKEN Brain Science Institute)  Itohara, ShigeYoshi 
Strain Status /
Availability
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