Strain Information | |
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Image | |
BRC No. | RBRC01923 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Zeb1<tm2Yhi>/YhiRbrc |
Former Common name | delta EF1 Knockout mice, delta EF1 null (lacZ), B6;129-Zfhx1a<tm2Yhi> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yujiro Higashi, Institute for Molecular and Cellular Biology, Osaka University. R1 ES cells were used. The mutant mice were crossed to C57BL/6. |
Strain description | Zeb1 gene knockout mice (deltaEF1 null lacZ). Exon 1 was replaced with a lacZ-neo cassette including termination signals. Homozygous mutant mice die shortly after birth. |
Colony maintenance | |
References | Development, 125, 21-31 (1998). 9389660 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Zeb1zinc finger E-box binding homeobox 118Zeb1<tm2Yhi>targeted mutation 2, Yujiro Higashidelta EF1, 3110032K11Rik, AREB6, MEB1, Nil2, Nil2, Tcf18, Tcf8, ZEB, Zfhep, Zfx1a, Zfhx1a Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)18SV40 early gene promoter and termination sequence (SV40), Thymidine kinase gene promoter sequence (HSV) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)18herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neomycin resistance gene coding sequence, E. coli LacZ gene coding sequence, SV40 early gene promoter and termination sequence, mouse Zeb1 genomic DNA |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 125, 21-31 (1998).The RECIPIENT should consult with the DEPOSITOR in the case of application for any patents or commercial use with the results derived from the use of the BIOLOGICAL RESOURCE. The RECIPIENT should consult with the DEPOSITOR on the authorship upon publications of the results obtained from the use of the BIOLOGICAL RESOURCE. The authorship should be considered in accordance to generally accepted practices and decided through agreement between the RECIPIENT and the DEPOSITOR/DEVELOPER. |
Depositor | Yujiro Higashi (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |