Strain Information | |
---|---|
Image | |
BRC No. | RBRC01926 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Vamp7<tm1Aha> |
Former Common name | Vamp7 KO, B6;129-Vamp7<tm1> |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Akihiro Harada, Institute for Molecular and Cellular Regulation, Gunma University in 2005. Two lines were generated (RBRC01926 and RBRC04855). |
Strain description | Vamp7 floxed mice (Neo type). Exons 3 and 4 of the Vamp7 gene were flanked by loxP sites, an FRT-SA-Ex 5,6,7,8-IRES-Neo-polyA-FRT cassette was inserted between exons 4 and 5. Vamp7 is a member of VAMP (vesicle-associated membrane protein) subfamily of SNARES proteins which primarily mediate fusion of cellular transport vesicles with a target membrane, and ubiquitously expressed and in localized to the late endsome, the lysosome, and the trans-Golgi network (TGN). |
Colony maintenance | Homozygote x HemizygoteHomozgous females and hemizygous males are viable and fertile. |
References | Mol. Biol. Cell, 9, 1437-1448 (1998). 9614185 Traffic., 12(10):1383-1393 (2011). 21740490 J. Cell Biol., 2000 May 15;149(4):889-900. 10811829 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xneo; neomycin; |
Ordering Information | |
---|---|
Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli neo, phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse VAMP7 genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concerning the analysis of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Akihiro Harada (Gunma University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
Xu H, Oses-Prieto JA, Khvotchev M, Jain S, Liang J, Burlingame A, Edwards RH. Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1. Nat Neurosci (2023) 37723322 |