Strain Data Sheet

RBRC01949

Strain Information

Image
BRC No.RBRC01949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4(Cg)-Tsix<tm1Sado>
Former Common nameTsix SA; Tsix splicing defect mutant mice
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2006. J1 ES cells were used to generate the mutant mice. Puromycin resistance was excised by crossing with cre transgenic mice.
Strain descriptionTsix gene splicing knockout mice (Tsix<SA>). The splicing acceptor site of Tsix exon 4 was replaced with a fragment containing an IRES-EGFP and a floxed puromycin resistance gene. This mutant line carries modified allele of Tsix locus to avoid the production major splicing variant. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
ReferencesDevelopment, 133, 4925-4931 (2006). 17108001

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TsixX (inactive)-specific transcript, antisenseXTsix<tm1Sado>targeted mutation 1, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)XEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, mouse Tsix genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 133, 4925-4931 (2006).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Feb 2009
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data