Strain Information | |
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Image | |
BRC No. | RBRC01950 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-Xist<tm1Sado> Tsix<tm1Enl> |
Former Common name | X<dc>; Xist/Tsix double KO mouse |
H-2 Haplotype | |
ES Cell line | J1 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Takashi Sado, National Institute of Genetics in 2005. |
Strain description | Xsit/Tsix double knockout mice (X<dc>). An IRES-EGFP cassette was inserted to replace part of exon 1 of Xist gene in mutated J1 ES cells lacking Tsix gene (Tsix<tm1Enl>) in which exon 2 and 3 were replaced with an IRES Beta-geo cassette. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). |
Colony maintenance | Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] |
References | Developmental Cell, 9, 159-165 (2005). 15992549 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)XEGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)X Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xneo; neomycin; |
Ordering Information | |
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Donor DNA | mouse Xist/Tsix genomic DNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Phage P1 loxP, E. coli neo, E. coli lacZ |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 9, 159-165 (2005).The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Takashi Sado (National Institute of Genetics) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Feb 2009 |
BRC mice in Publications |
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No Data |