Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2018
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RBRC No. RBRC01965  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6;129S4-Fbxo15<tm1Yam>  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line RF8 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times.  
Strain description Fbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undiffereniated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression. Homozygous mutant mice develop normally and are fertile.  
Colony maintenance Sibling Mating (Homozygote x Homozygote)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Fbxo15  
Symbol name F-box protein 15  
Chromosome 18  
Common name ecat3, Fbx15  
Symbol description No Data  
 2 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 18  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 18  
Common name neo; neomycin;  
Symbol description No Data  
 4 Symbol No Data  
Symbol name SV40 poly A  
Chromosome 18  
Common name No Data  
Symbol description No Data  
 5 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 18  
Common name No Data  
Symbol description E. coli beta galactosidase  
References Mol Cell Biol. 2003 Apr;23(8):2699-708.  
Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development.
Research applications Developmental Biology Research  
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.  
Additional information
 1 MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English (Word)  
 2 Genotyping protocol <PCR>  
Depositor Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university)  Yamanaka, Shinya 
Strain Status /
Availability
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