Strain Information | |
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Image | |
BRC No. | RBRC01966 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129S4-ERas<tm1Yam> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | |
Appearance | |
Strain development | Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times. |
Strain description | ERas gene is important in the tumor-like grouwth properties of ES cells.Homozygous mutant mice develop normally and are fertile. |
Colony maintenance | Sibling Mating (Homozygote x Homozygote) |
References | Nature, 423, 541-545 (2003). 12774123 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ErasES cell-expressed RasXEras<tm1Yam>targeted mutation 1, Shinya Yamanakaecat5, Ha-Ras2, HRAS2, HRasp Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)Xinternal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)X Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xneo; neomycin; Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 pAX |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, E. coli neo, SV40 pA, mouse ERas genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | |
Depositor | Shinya Yamanaka (Kyoto University) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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No Data |