|
RBRC No. |
RBRC01966
|
|
Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
B6;129S4-ERas<tm1Yam> |
|
Former Common name |
No Data |
|
H-2 Haplotype |
No Data |
|
ES cell line |
RF8 [129S4/SvJae] |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
No Data |
| Genotype |
No Data |
|
Strain development |
Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times. |
|
Strain description |
ERas gene is important in the tumor-like grouwth properties of ES cells.
Homozygous mutant mice develop normally and are fertile. |
|
Colony maintenance |
Sibling Mating (Homozygote x Homozygote) |
|
Health Report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Eras |
| Symbol name |
ES cell-expressed Ras |
| Chromosome |
X |
| Common name |
ecat5, Ha-Ras2, HRAS2, HRasp |
| Symbol description |
No Data |
| 2 |
Symbol |
IRES |
| Symbol name |
internal ribosomal entry site (EMCV) |
| Chromosome |
X |
| Common name |
internal ribosomal entry site from EMCV |
| Symbol description |
No Data |
| 3 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
X |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
| 4 |
Symbol |
lacZ |
| Symbol name |
beta-galactosidase (E. coli) |
| Chromosome |
X |
| Common name |
No Data |
| Symbol description |
E. coli beta galactosidase |
|
References |
Nature. 2003 May 29;423(6939):541-5. Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. |
|
Research applications |
No Data |
|
Specific Term and Conditions |
For inquiries please mail us at animal@brc.riken.jp. |
|
Additional information |
|
1 |
MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English (Word) |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university)  |
|
Strain Status /
Availability
(Expected delivery) |
|
