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RBRC No. |
RBRC01967
|
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Type |
Targeted Mutation |
 |
|
Species |
Mus musculus |
|
Strain name |
B6;129S4-Nanog<tm1Yam> |
|
Former Common name |
No Data |
|
H-2 Haplotype |
No Data |
|
Background strain |
No Data |
|
Appearance |
| 1 |
Appearance |
No Data |
| Genotype |
No Data |
|
Strain development |
Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exson 2 whcich contains the homeodomain. The mutant mice were backcross to C57BL/6J. |
|
Strain description |
Nanog gene is a important factor of maintaining ES cell pluripotent differentiation.
Homozygous mutant mice are embryonic lethal. |
|
Colony maintenance |
Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
|
Animal health report |
|
|
Gene Details |
|
Promoter |
No Data |
| 1 |
Symbol |
Nanog |
| Symbol name |
Nanog homeobox |
| Chromosome |
6 |
| Common name |
2410002E02Rik, ecat4, ENK |
| Symbol description |
No Data |
| 2 |
Symbol |
IRES |
| Symbol name |
internal ribosomal entry site (EMCV) |
| Chromosome |
6 |
| Common name |
internal ribosomal entry site from EMCV |
| Symbol description |
No Data |
| 3 |
Symbol |
neo |
| Symbol name |
neomycin resistance gene (E. coli) |
| Chromosome |
6 |
| Common name |
neo; neomycin; |
| Symbol description |
No Data |
| 4 |
Symbol |
lacZ |
| Symbol name |
beta-galactosidase (E. coli) |
| Chromosome |
6 |
| Common name |
No Data |
| Symbol description |
E. coli beta galactosidase |
|
References |
Cell. 2003 May 30;113(5):631-42. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. |
|
Research applications |
Developmental Biology Research |
|
Specific Term and Conditions |
For inquiries please mail us at animal@brc.riken.jp. |
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Additional information |
|
1 |
MATERIAL TRANSFER AGREEMENT (Japanese / English) |
|
2 |
Genotyping protocol <PCR> |
|
Depositor |
Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university)  |
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Strain Status /
Availability
(Expected delivery) |
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