Strain Information | |
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Image | |
BRC No. | RBRC01968 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S4-Visfatin<tm1Yam> |
Former Common name | |
H-2 Haplotype | |
ES Cell line | RF8 [129S4/SvJae] |
Background strain | C57BL/6JJcl |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2001. The mutant mice were backcross to C57BL/6J. |
Strain description | Visfatin is produced by the visceral fat and whose expression level in plasma increases during the development of obesity. Visfatin exterted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. Homozygous mutant mice are embryonic lethal. Heterozygous mice show increased plasma glucose levels but normal insulin evels. |
Colony maintenance | Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl) |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Namptnicotinamide phosphoribosyltransferase12Nampt<tm1Yam>targeted mutation 1, Shinya Yamanaka1110035O14Rik, Visfatin, Pbef1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)12En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)12internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)12 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)12neo; neomycin; |
Ordering Information | |
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Donor DNA | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocorditis virus (EMCV) internal ribosomal entrysite (IRES), E. coli neo, E. coli lacZ, SV40 poly A, mouse Visfatin genomic DNA |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall conclude the Memorandum of Understanding with KYOTO UNIVERSITY (contact KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp) before receiving the ORIGINAL MATERIAL. |
Depositor | Shinya Yamanaka (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |