Strain Data Sheet
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Data update: Aug 20, 2018
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RBRC No. RBRC01969  
Type Targeted Mutation and TransgeneCartagena  
Species Mus musculus  
Strain name STOCK Tg(CAG-EGFP)1Yam Fbxo15tm1Yam  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line RF8 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Shinya Yamanaka at Nara Institute of Science and Technology in 2004. Mixed background. KO: Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the exon 3 through 7. The mutant mice were backcross to C57BL/6J for several times. Tg: The EGFP under the CAG promoter was injected into the pronuclei of fertilized eggs. ICR background.  
Strain description Fbxo15 knockout and CAG-EGFP transgenic double mutant mice. Fbxo15, F-box protein 15 is a novel target of undifferentiated-cell-specific transcripton factor Oct3/4 playing essential roles in self-renewal of ES cells and mouse early development. Fbxo15 is predominantly expressed in mouse undifferentiated ES cells. Inactivation of Oct3/4 in ES cells lead to rapid disappearance of Fbxo15 expression.  
Colony maintenance Tg: Carrier x Noncarrier; KO: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Fbxo15  
Symbol name F-box protein 15  
Chromosome 18  
Common name ecat3, Fbx15  
Symbol description No Data  
 2 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 18  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 18  
Common name neo; neomycin;  
Symbol description No Data  
 4 Symbol No Data  
Symbol name SV40 polyA  
Chromosome 18  
Common name No Data  
Symbol description No Data  
 5 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 18  
Common name No Data  
Symbol description E. coli beta galactosidase  
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)  
 6 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Cell. 2006 Aug 25;126(4):663-76. Epub 2006 Aug 10.
Mol Cell Biol. 2003 Apr;23(8):2699-708.  
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Research applications Developmental Biology Research  
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.  
Additional information
 1 MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English (Word)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
Depositor Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university)  Yamanaka, Shinya 
Strain Status /
Availability
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