Strain Data Sheet

RBRC02087

Strain Information

Image
BRC No.RBRC02087
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Pcdha<tm(AdAB)> Olfr17<tm1Mom>/TyagRbrc
Former Common nameP2-LacZ/AdAB
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR AdAB (RBRC01687) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University) and P2-lacZ (Peter Monbaerts, The Rockefeller University) mice.
Strain descriptionCNR (Pcdha) AdAB mutant (CR1, CR2 and CR3a were deleted) and P2 (Olfr17) gene ires-taulacZ knock-in double mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; Olfr17: Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesCell, 87, 675-686 (1996). 8929536
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm(AdAB)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Olfr17olfactory receptor 177Olfr17<tm1Mom>targeted mutation 1, Peter Mombaerts

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNA7tau

Ordering Information

Donor DNAphage P1 loxP site, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), bovine tau cDNA, E.coli lacZ, mouse Pcdha genomic DNA, mouse Olfr17 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data