Strain Data Sheet

RBRC02139

Strain Information

Image
BRC No.RBRC02139
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Cnga2<tm1Mom> Olfr151<tm25Mom> Pcdha<tm3Tyag>/TyagRbrc
Former Common nameOCNC1/M71-LacZ/BdAB
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. The mutant mice were generated by crossing with CNR delta CR (BdAB) (RBRC01688) (Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University) and OCNC1 KO and M71-lacZ mice (Peter Monbaerts, The Rockefeller University).
Strain descriptionOCNC1 (Cnga2) knockout, CNR (Pcdha) delta CR mutant and M71 (Olfr151) gene ires-taulacZ knock-in triple mutant mice.
Colony maintenancePcdha: Homozygote x Homozygote; Olfr151: Homozygote x Homozygote; Cnga2: Heterozygote (female) x Wild-type (male)
ReferencesNeuron, 35, 681-696 (2002). 12194868
Neuron. 26(1):81-91 (2000). 10798394
Mol Cell Neurosci, 38, 66-79 (2008). 18353676

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm3Tyag>targeted mutation 3, Takeshi YagiA830022B16Rik, Cnr7, Crnr7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Olfr151olfactory receptor 1519Olfr151<tm25Mom>targeted mutation 25, Peter MombaertsGA_x6K02T2PVTD-31408136-31407207, M71, MOR171-2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNA9tau

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cnga2cyclic nucleotide gated channel alpha 2XCnga2<tm1Mom>targeted mutation 1, Peter MombaertsCnca, Cncg4, OCNC1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, bovine tau cDNA, mouse Olfr151 genomic DNA, mouse Pcdha genomic DNA, mouse Cnga2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior consent on use of it from Professor Peter Monbaerts, The Rockefeller University. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate the treatment of such invention. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. The RECIPIENT, which wishes to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data