Strain Data Sheet

RBRC02216

Strain Information

Image
BRC No.RBRC02216
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(nodalNDE-hsp68-lacZ)H11Hmd
Former Common nameNodal NDE lacZ (H11)
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C (not fixed yet)]
agouti [A/? B/B C/C (not fixed yet)]
Strain developmentDeveloped by Drs. Naomi Iwai and Hiroshi Hamada at Oasaka University in 2001.
Strain descriptionThis transgenic mice carry a hsp68-lacZ gene under control of a 0.8-kb region upstream of Nodal coding sequences (-9.5 and -8.7 kb) containing the node-specific enhancer (NDE). X-gal staining around the node can be detected in embryos at early somite stages.
Colony maintenanceCarrier x Noncarrier or Crossing to Jcl:B6C3F1Tg mice are viable and fertile.
ReferencesGenes Dev.,17, 1207-1212 (2003). 12730124

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UNmouse Nodal enhancer/hsp68 promoter

Ordering Information

Donor DNAE. coli lacZ, mouse nodal node-specific expression enhancer, hsp68 promoter
Research applicationDevelopmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev.,17, 1207-1212 (2003).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data