Strain Data Sheet
RBRC02231
Strain Information | |
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| Image | |
| BRC No. | RBRC02231 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129-Atg5<tm1Nmz> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Masahiko Hatano and Takeshi Tokuhisa, Graduate School of Medicine, Chiba University, and Noboru Mizushima, National Institute for Basic Biology in 2003. The knockout construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J. |
| Strain description | Atg5 knockout mice. Exons 1 and 2 of Atg5 were replaced with a neomycin resistance gene. Homozygous mutant mice die during the early neonatal period due to a lack of nutrients. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional mice (floxed allele) are also available: B6.129S-Atg5<tm1Myok> (RBRC02975). |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc] |
| References | Nature, 432, 1032-1036 (2004) 15525940 |
Health Report | |
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| Examination Date / Room / Rack | 2022/05/23Room:4-ARack:D |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Atg5autophagy-related 5 (yeast)10Atg5<tm1Nmz>targeted mutation 1, Noboru Mizushima2010107M05Rik, 3110067M24Rik, Apg5l, Paddy Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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| Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Atg5 genomic DNA |
| Research application | Cell Biology Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 432, 1032-1036 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Noboru Mizushima (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background Mouse of the Month Jul 2009 |
BRC mice in Publications |
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Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 140(3) 313-26(2010) 20144757 Najafov A, Luu HS, Mookhtiar AK, Mifflin L, Xia HG, Amin PP, Ordureau A, Wang H, Yuan J. RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Mol Cell 81(2) 370-385.e7(2021) 33271062 Wu Y, Li Y, Zhang H, Huang Y, Zhao P, Tang Y, Qiu X, Ying Y, Li W, Ni S, Zhang M, Liu L, Xu Y, Zhuang Q, Luo Z, Benda C, Song H, Liu B, Lai L, Liu X, Tse HF, Bao X, Chan WY, Esteban MA, Qin B, Pei D. Autophagy and mTORC1 regulate the stochastic phase of somatic cell reprogramming. Nat Cell Biol 17(6) 715-25(2015) 25985393 |