Strain Data Sheet
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Data update: Oct 24, 2018
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RBRC No. RBRC02290  
Type TransgeneCartagena   wks
Species Mus musculus  
Strain name STOCK Tg(Nanog-GFP,Puro)1Yam  
Former Common name No Data  
H-2 Haplotype No Data  
ES cell line RF8 [129S4/SvJae]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Shinya Yamanaka, Institute for Frontier Medical Sciences, Kyoto University in 2003. The construct was injected into RF8 ES cells derived from 129S4/SvJae. 129, C57BL/6 and DBA/2 mixed background.  
Strain description Nanog-GFP transgenic mice. Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). This Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5' untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency. Tg homozygous and hemizygous mice were viable and fertile. Male-biased sex ratios was observed (199 females : 261 males)(details unknown).  
Colony maintenance Carrier x Carrier (Homozygote x Homozygote)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 3 Symbol GH  
Symbol name Growth hormone polyA (Bovine)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol puro  
Symbol name puromycin resistance gene (Streptomyces alboniger)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
Promoter Nanog promoter  
 5 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References Nature. 2007 Jul 19;448(7151):313-7. Epub 2007 Jun 6.  
Generation of germline-competent induced pluripotent stem cells.
Research applications Cell Biology Research,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.  
Additional information
 1 Mouse of the Month Dec 2007
Mouse of the Month Oct 2012  
 2 Distribution of the iPS cells (Japanese/ English)
MATERIAL TRANSFER AGREEMENT (Japanese / English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 3 Genotyping protocol <PCR>  
Depositor Yamanaka, Shinya (Center for iPS Cell Research and Application, Kyoto University)  Yamanaka, Shinya 
Strain Status /
Availability
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