Strain Data Sheet

RBRC02295

Strain Information

Image
BRC No.RBRC02295
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common nameCas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain descriptionCasp130 (Bcar1) gene exon 2 floxed and BLG (beta lactoglobulin)-cre double mutant mice. Viable and fertile, but the reproductive performance is not so good.
Colony maintenanceKO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
ReferencesHepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm1.2Homy>targeted mutation 1.2, HomyCas, Crkas, p130 Cas

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcresheep BLG promoter

Ordering Information

Donor DNAphage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data