Strain Information | |
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Image | |
BRC No. | RBRC02298 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C.Cg-Il3<tm1Glli> |
Former Common name | IL-3 knock-out mouse |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | BALB/cAnNTac |
Appearance | albino [A/A b/b c/c] |
Strain development | Developed by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. |
Strain description | Interluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised. |
Colony maintenance | Backcross to BALB/c (Heterozygote x BALB/cAnNTac) |
References | Blood, 91, 778-783 (1998). 9446636 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Amouse PGK polyA11poly A |
Ordering Information | |
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Donor DNA | mouse Il3 genomic DNA, mouse Phosphoglycerate kinase-1(PGK) promoter, E. coli neo, mouse PGK polyA |
Research application | Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Glenn Dranoff, MD, Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu). |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Bénard A, Mittelstädt A, Klösch B, Glanz K, Müller J, Schoen J, Nüse B, Brunner M, Naschberger E, Stürzl M, Mattner J, Muñoz LE, Sohn K, Grützmann R, Weber GF. IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils. Cell Rep 42(6) 112637(2023) 37300834 Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M. Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation. Immunity 40(5) 758-71(2014) 24837103 Balam S, Schiechl-Brachner G, Buchtler S, Halbritter D, Schmidbauer K, Talke Y, Neumayer S, Salewski JN, Winter F, Karasuyama H, Yamanishi Y, Renner K, Geissler EK, Mack M. IL-3 Triggers Chronic Rejection of Cardiac Allografts by Activation of Infiltrating Basophils. J Immunol 202(12) 3514-3523(2019) 31068389 Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M. Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade. Nat Commun 4 2847(2013) 24284909 Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönnchen T, Rahbari NN, Schölch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK. Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis. Science 347(6227) 1260-5(2015) 25766237 Julia Ko¨lle, Theodor Zimmermann, Alexander Kiefer, Ralf J. Rieker, Paraskevi Xepapadaki, Sebastian Zundler, Nikolaos G. Papadopoulos, Susetta Finotto Targeted deletion of Interleukin-3 results in asthma exacerbations iScience 104440(2022) 35707726 |