Strain Information | |
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Image | |
BRC No. | RBRC02302 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Lyzs<tm1(cre)Ifo> |
Former Common name | LysM-Cre knock-in mice (C57BL/6), B6.129P2-Lyzs<tm1(cre)Ifo> |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6NTac |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303). |
Strain description | Lyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6NTac] |
References | Transgenic Res., 8, 265-277 (1999) 10621974 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-5Rack:A 2023/12/11Room:3-5Rack:A 2023/09/11Room:3-5Rack:A 2023/06/13Room:3-5Rack:A 2023/03/13Room:3-5Rack:A 2022/12/12Room:3-5Rack:A 2022/09/12Room:3-5Rack:A 2022/06/13Room:3-5Rack:A |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Lyz2lysozyme 210Lyz2<tm1(cre)Ifo>targeted mutation 1, Irmgard FoersterLC, Lys-cre, LysM Cre, lysM/cre, LysMCre, LysMcre, Lyzs Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase10cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)10nls |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), Phage P1 cre, yeast FRT (flipase recombination target) site, mouse lysozyme M genomic DNA |
Research application | Cell Biology Research Cre/loxP system FLP/frt system Immunology and Inflammation Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de. |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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Sato H, Taketomi Y, Miki Y, Murase R, Yamamoto K, Murakami M. Secreted Phospholipase PLA2G2D Contributes to Metabolic Health by Mobilizing ω3 Polyunsaturated Fatty Acids in WAT. Cell Rep 31(5) 107579(2020) 32375030 Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K. IRF4 in dendritic cells inhibits IL-12 production and controls Th1 immune responses against Leishmania major. J Immunol 192(5) 2271-9(2014) 24489086 Mitsui K, Hishiyama S, Jain A, Kotoda Y, Abe M, Matsukawa T, Kotoda M. Role of macrophage autophagy in postoperative pain and inflammation in mice. J Neuroinflammation 20(1) 102(2023) 37131209 Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I. Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever. J Neurosci 38(24) 5584-5595(2018) 29899035 |