Strain Data Sheet

RBRC02308

Strain Information

Image
BRC No.RBRC02308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cxcl14<tm1>
Former Common nameB6BRAKwt/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Ryu-Ichiro Hata, Kanagawa Dental College in 2008.
Strain descriptionC57BL/6, Cxcl14(BRAK) gene exon2 was substituted with floxp neo gene
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type)
ReferencesBiochem. BIophys. Res. Commun., 348, 406-412 (2006). 16884687
Inflamm. Regen., 30, 536-541 (2010).

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcl14chemokine (C-X-C motif) ligand 1413Cxcl14<tm1Tha>targeted mutation 1, Takahiko Hara1110031L23Rik, 1200006I23Rik, bolekine, BRAK, Kec, KS1, MIP-2g, Scyb14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Cxcl14 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The first paper making use of the donated mouse must be collaboration with the DEPOSITOR.
DepositorRyu-Ichiro Hata (Kanagawa Dental College)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data