Strain Data Sheet
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Data update: Jul 7, 2014
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RBRC No. RBRC02370  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6;129-Grn<tm1>  
Former Common name Progranulin knock-out mouse  
H-2 Haplotype No Data  
Background strain C57BL/6  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd.  
Strain description Grn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male.  
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc].  
Health Certificate
Gene Details
Promoter No Data  
 1 Symbol Grn  
Symbol name granulin  
Chromosome 11  
Common name acrogranulin, epithelin, PC cell-derived growth factor, progranulin  
Symbol description No Data  
 2 Symbol poly A  
Symbol name bovine growth hormone poly adenylation signal sequence  
Chromosome 11  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 11  
Common name neo; neomycin;  
Symbol description No Data  
References Behav Brain Res. 2007 Jul 20;.  
Alteration of behavioural phenotype in mice by targeted disruption of the progranulin gene.
Research applications Neurobiology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Behav. Brain. Res., 185, 110-118 (2007).
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.  
Additional information
 1 Genetic Background  
 2 Genotyping protocol <PCR>  
Depositor Nishihara, Masugi (The University of Tokyo)  Nishihara, Masugi 
Strain Status /
Availability
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