Strain Data Sheet

RBRC02397

Strain Information

Image
BRC No.RBRC02397
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Pknox2<tm1Nuk>
Former Common namePrep2LacZKI, C57BL/6
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Dr. Atsushi Kuroiwa, Graduate School of Science, Nagoya University. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette.
Strain descriptionPrep2(Pknox2) is a transcriptional regulator having TALE momeodomain, and is involved in transcriptinal regulation by binding to the target sequence as a heterodimer with transcription factor of Pbx having TALE homeodomain. lacZ-SV40 poly A-loxP-PGK-neo-poly A loxP. In frame insertion of a lacZ gene with a SV40 poly A into the first coding exon followed by a floxed PGK-neo cassette.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pknox2Pbx/knotted 1 homeobox 29Pknox2<tm1Nuk>targeted mutation 1, Nagoya University Atsushi KuroiwaD230005H23Rik, Prep2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amouse pgk polyA9poly A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA9poly A

Ordering Information

Donor DNAphage P1 loxP sites, mouse Pgk promoter sequence, E. coli neomycin resistance gene, mouse Pgk polyA additional signal, E. coli LacZ, SV40 polyA additional signal sequence, mouse Pknox2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorAtsushi Kuroiwa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data